mouse crp elisa kit Search Results


mouse c reactive protein crp quantikine elisa kit  (R&D Systems)
93
R&D Systems mouse c reactive protein crp quantikine elisa kit
Effect of B. coagulans spores, PSCF and synbiotic on immune markers in colon tissues and blood serum. Protein levels of cytokines including ( A ) IL-1α, ( B ) IL-1β, ( C ) IL-6, ( D ) IL-12, ( E ) TNF-α, ( F ) IFN-γ in proximal and distal colon explants as well as cytokine levels of ( G ) IL-1β, ( H ) IL-10, and ( I ) IL-12 in blood serum were analysed by Bio-plex. iNOS activity in colon tissues ( J ) measured by NOS activity assay and CRP levels in serum ( K ) by <t>ELISA.</t> Statistical significance among groups evaluated by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. DSS-colitic group and data expressed as mean ± SEM ( n = 3 per group).
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crp  (Multi Sciences (Lianke) Biotech Co Ltd)
94
Multi Sciences (Lianke) Biotech Co Ltd crp
( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together <t>with</t> <t>IFN-γ</t> and <t>CRP</t> in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.
Crp, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csb e07923m  (Cusabio)
90
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( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together <t>with</t> <t>IFN-γ</t> and <t>CRP</t> in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.
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c reactive protein  (R&D Systems)
93
R&D Systems c reactive protein
( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together <t>with</t> <t>IFN-γ</t> and <t>CRP</t> in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.
C Reactive Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse crp elisa kit  (Boster Bio)
92
Boster Bio mouse crp elisa kit
( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together <t>with</t> <t>IFN-γ</t> and <t>CRP</t> in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.
Mouse Crp Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse c reactive protein crp assaymax elisa kit  (Assaypro)
91
Assaypro mouse c reactive protein crp assaymax elisa kit
( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together <t>with</t> <t>IFN-γ</t> and <t>CRP</t> in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.
Mouse C Reactive Protein Crp Assaymax Elisa Kit, supplied by Assaypro, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse c reactive protein elisa quantitation kit  (Aviva Systems)
86
Aviva Systems mouse c reactive protein elisa quantitation kit
( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together <t>with</t> <t>IFN-γ</t> and <t>CRP</t> in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.
Mouse C Reactive Protein Elisa Quantitation Kit, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio plex pro mouse cytokine assay  (Assaypro)
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Assaypro bio plex pro mouse cytokine assay
( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together <t>with</t> <t>IFN-γ</t> and <t>CRP</t> in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.
Bio Plex Pro Mouse Cytokine Assay, supplied by Assaypro, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse saa elisa kit  (Immunology Consultants Laboratory Inc)
90
Immunology Consultants Laboratory Inc mouse saa elisa kit
( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together <t>with</t> <t>IFN-γ</t> and <t>CRP</t> in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.
Mouse Saa Elisa Kit, supplied by Immunology Consultants Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse high-sensitive crp elisa kit  (Kamiya)
90
Kamiya mouse high-sensitive crp elisa kit
Bcl-2 expression in monocytes results in reduced inflammatory cells infiltration in the hearts of MCP mice. (A) Representative photomicrographs demonstrating immunohistochemical staining with anti-CD45, -Mac-1 and -Mac-3 antibodies in heart sections from 6-month-old wild-type and transgenic mice. Positive-stained cells were visualized with diaminobenzidine (brown). (B–D) Histograms showing the number of CD45-, Mac-1- and Mac-3-positive cells in the hearts of wild-type, MCP and MCP/Bcl-2 mice. *P <0.001 versus wild-type mice; #P <0.05 versus wild-type and MCP mice; n =6 per group per time point. (E) Circulating levels of <t>CRP</t> assayed by <t>ELISA.</t> n =6 per group per time point. *P <0.05 versus wild-type and MCP/Bcl-2 mice; #P <0.05 versus wild-type mice.
Mouse High Sensitive Crp Elisa Kit, supplied by Kamiya, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse c-reactive protein (crp) elisa kit ls-f4264  (Absolute Biotech Inc)
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Bcl-2 expression in monocytes results in reduced inflammatory cells infiltration in the hearts of MCP mice. (A) Representative photomicrographs demonstrating immunohistochemical staining with anti-CD45, -Mac-1 and -Mac-3 antibodies in heart sections from 6-month-old wild-type and transgenic mice. Positive-stained cells were visualized with diaminobenzidine (brown). (B–D) Histograms showing the number of CD45-, Mac-1- and Mac-3-positive cells in the hearts of wild-type, MCP and MCP/Bcl-2 mice. *P <0.001 versus wild-type mice; #P <0.05 versus wild-type and MCP mice; n =6 per group per time point. (E) Circulating levels of <t>CRP</t> assayed by <t>ELISA.</t> n =6 per group per time point. *P <0.05 versus wild-type and MCP/Bcl-2 mice; #P <0.05 versus wild-type mice.
Mouse C Reactive Protein (Crp) Elisa Kit Ls F4264, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse c-reactive protein kit  (Life Diagnostics Inc)
90
Life Diagnostics Inc mouse c-reactive protein kit
Bcl-2 expression in monocytes results in reduced inflammatory cells infiltration in the hearts of MCP mice. (A) Representative photomicrographs demonstrating immunohistochemical staining with anti-CD45, -Mac-1 and -Mac-3 antibodies in heart sections from 6-month-old wild-type and transgenic mice. Positive-stained cells were visualized with diaminobenzidine (brown). (B–D) Histograms showing the number of CD45-, Mac-1- and Mac-3-positive cells in the hearts of wild-type, MCP and MCP/Bcl-2 mice. *P <0.001 versus wild-type mice; #P <0.05 versus wild-type and MCP mice; n =6 per group per time point. (E) Circulating levels of <t>CRP</t> assayed by <t>ELISA.</t> n =6 per group per time point. *P <0.05 versus wild-type and MCP/Bcl-2 mice; #P <0.05 versus wild-type mice.
Mouse C Reactive Protein Kit, supplied by Life Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of B. coagulans spores, PSCF and synbiotic on immune markers in colon tissues and blood serum. Protein levels of cytokines including ( A ) IL-1α, ( B ) IL-1β, ( C ) IL-6, ( D ) IL-12, ( E ) TNF-α, ( F ) IFN-γ in proximal and distal colon explants as well as cytokine levels of ( G ) IL-1β, ( H ) IL-10, and ( I ) IL-12 in blood serum were analysed by Bio-plex. iNOS activity in colon tissues ( J ) measured by NOS activity assay and CRP levels in serum ( K ) by ELISA. Statistical significance among groups evaluated by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. DSS-colitic group and data expressed as mean ± SEM ( n = 3 per group).

Journal: Nutrients

Article Title: Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD

doi: 10.3390/nu11040818

Figure Lengend Snippet: Effect of B. coagulans spores, PSCF and synbiotic on immune markers in colon tissues and blood serum. Protein levels of cytokines including ( A ) IL-1α, ( B ) IL-1β, ( C ) IL-6, ( D ) IL-12, ( E ) TNF-α, ( F ) IFN-γ in proximal and distal colon explants as well as cytokine levels of ( G ) IL-1β, ( H ) IL-10, and ( I ) IL-12 in blood serum were analysed by Bio-plex. iNOS activity in colon tissues ( J ) measured by NOS activity assay and CRP levels in serum ( K ) by ELISA. Statistical significance among groups evaluated by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. DSS-colitic group and data expressed as mean ± SEM ( n = 3 per group).

Article Snippet: The levels of C-reactive protein (CRP) in serum from respective groups ( n = 3 samples/group) were analysed using Mouse C-Reactive Protein/CRP Quantikine Elisa kit (MCRP00, R and D Systems, Australia) following the manufacturer’s instructions.

Techniques: Activity Assay, Nos Activity Assay, Enzyme-linked Immunosorbent Assay

( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together with IFN-γ and CRP in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.

Journal: Science Advances

Article Title: Versatility of bacterial outer membrane vesicles in regulating intestinal homeostasis

doi: 10.1126/sciadv.ade5079

Figure Lengend Snippet: ( A to D ) In vivo effects on repairing the gut physicochemical barrier of DSS mice after daily oral delivery of 100 μl of Akk OMV suspension containing 20 μg of total proteins or PBS for 5 days. (A) Alcian blue staining images of the proximal colon. Black and yellow arrows represent goblet cells with pathological cavities and the thickened mucus layer, respectively. Scale bars, 600 μm. (B) Percentage of goblet cells in the intestinal epithelium. (C) 3D CLSM images of the intestinal epithelial cells. Red fluorescence indicates Cy5.5-labeled Akk OMVs. Scale bars, 50 μm. (D) Immunofluorescence images of tight junctions ZO-1 and occludin expressed on the epithelial cells in the proximal colon. Scale bars, 20 μm. ( E and F ) In vitro effects on Caco-2 cells after treatment with Akk OMVs (0.1 mg/ml) along with LPS (5 μg/ml) at 37°C for 24 hours. Cy5.5-labeled OMVs were used for CLSM. (E) Immunofluorescence and (F) CLSM images. Scale bars, 25 μm. ( G ) Cytokine levels of IL-10, IL-13, and ACE2 in colon tissue together with IFN-γ and CRP in serum measured by ELISA. Samples were collected from DSS mice after treatment with Akk OMVs or PBS. Data are means ± SEM ( n ≥ 5). Significance was assessed using t test, giving P values: * P < 0.05.

Article Snippet: Levels of cytokines including IL-10 (EK210/4, MultiSciences), IL-13 (EK213/2, MultiSciences), and ACE2 (EK1188, Boster Biological Technology) in colon tissue as well as IFN-γ (EK280/3, MultiSciences) and CRP (EK294/2, MultiSciences) in serum were measured by commercially available ELISA kits.

Techniques: In Vivo, Suspension, Staining, Fluorescence, Labeling, Immunofluorescence, In Vitro, Enzyme-linked Immunosorbent Assay

Bcl-2 expression in monocytes results in reduced inflammatory cells infiltration in the hearts of MCP mice. (A) Representative photomicrographs demonstrating immunohistochemical staining with anti-CD45, -Mac-1 and -Mac-3 antibodies in heart sections from 6-month-old wild-type and transgenic mice. Positive-stained cells were visualized with diaminobenzidine (brown). (B–D) Histograms showing the number of CD45-, Mac-1- and Mac-3-positive cells in the hearts of wild-type, MCP and MCP/Bcl-2 mice. *P <0.001 versus wild-type mice; #P <0.05 versus wild-type and MCP mice; n =6 per group per time point. (E) Circulating levels of CRP assayed by ELISA. n =6 per group per time point. *P <0.05 versus wild-type and MCP/Bcl-2 mice; #P <0.05 versus wild-type mice.

Journal:

Article Title: Monocyte-specific Bcl-2 expression attenuates inflammation and heart failure in monocyte chemoattractant protein-1 (MCP-1)-induced cardiomyopathy ⋆

doi: 10.1016/j.cardiores.2006.03.008

Figure Lengend Snippet: Bcl-2 expression in monocytes results in reduced inflammatory cells infiltration in the hearts of MCP mice. (A) Representative photomicrographs demonstrating immunohistochemical staining with anti-CD45, -Mac-1 and -Mac-3 antibodies in heart sections from 6-month-old wild-type and transgenic mice. Positive-stained cells were visualized with diaminobenzidine (brown). (B–D) Histograms showing the number of CD45-, Mac-1- and Mac-3-positive cells in the hearts of wild-type, MCP and MCP/Bcl-2 mice. *P <0.001 versus wild-type mice; #P <0.05 versus wild-type and MCP mice; n =6 per group per time point. (E) Circulating levels of CRP assayed by ELISA. n =6 per group per time point. *P <0.05 versus wild-type and MCP/Bcl-2 mice; #P <0.05 versus wild-type mice.

Article Snippet: The circulating levels of circulating C-reactive protein (CRP) in plasma were measured with mouse high-sensitive CRP ELISA kit (Kamiya Biomedical Company, Seattle, WA), which specifically detects mouse CRP.

Techniques: Expressing, Immunohistochemical staining, Staining, Transgenic Assay, Enzyme-linked Immunosorbent Assay